<b&bt;Flexible Formatting & Manipulation<-b&bt;

DNA can be represented and manipulated in either graphical or sequence-text modes - with any formatting or sequence manipulation changes made in one view, automatically being applied to the selection in the alternate view. Users have complete control over the display in either mode. Segments can be selected in either mode and cut, copied, or pasted. When a selected segment is cut or copied, the ends are defined by the sites used to delimit the segment. Gene Construction Kit automatically monitors sticky ends that can be generated by restriction enzymes. Incompatible ends cannot inadvertently be pasted together. If such an attempt is made, GCK utilizes a unique "ligation dialog" to assist users in adjusting the ends for compatibility. Utilizing the special paste option allows a sequence to be inverted and-or to have the ends filled or trimmed before pasting, thus circumventing the ligation dialog if the manipulations warrant it.
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